Publications by authors named "S M Handley"
Ross River virus (RRV) and other alphaviruses cause chronic musculoskeletal syndromes that are associated with viral persistence, which suggests deficits in immune clearance mechanisms, including CD8 T-cell responses. Here, we used a recombinant RRV-gp33 that expresses the immunodominant CD8 T-cell epitope of lymphocytic choriomeningitis virus (LCMV) to directly compare responses with a virus, LCMV, that strongly induces antiviral CD8 T cells. After footpad injection, we detected fewer gp33-specific CD8 T cells in the draining lymph node (DLN) after RRV-gp33 than LCMV infection, despite similar viral RNA levels in the foot.
View Article and Find Full Text PDFGenomic surveillance of SARS-CoV-2 in Nepal.
Binod Khadka Rajindra Napit Kathie A Mihindukulasuriya Smita Shrestha Ramanuj Rauniyar Scott A Handley
Microbiol Resour Announc
December 2024
Article Synopsis
- During the COVID-19 pandemic, Nepal encountered new variants of the SARS-CoV-2 virus.
- A study sequenced 278 samples from September 2021 to March 2022, resulting in 229 high-quality genomes.
- The findings revealed that 82.97% of the genomes were Omicron variants, while 17.03% were Delta variants, showcasing the genomic diversity of the virus in Nepal.
Stability of Antibiotics for Use in the Testing of Immediate Drug Allergy Reactions.
J Allergy Clin Immunol Pract
November 2024
Article Synopsis
- Limited information exists about the stability of penicillin-based antibiotics for skin testing, prompting a study on 16 parenteral antibiotics to evaluate their physicochemical stability and provide guidelines for clinicians.
- Most antibiotics showed good stability for 2 to 7 days, with amoxicillin/clavulanic acid being a notable exception, dropping below acceptable levels by day 2.
- The research included specific tests for chemical and physical stability, revealing that while many antibiotics maintained acceptable performance, some experienced significant color changes and pH drops over time.