GLC and NMR analysis of isomeric impurities in the new anti-inflammatory agent benoxaprofen.

GLC and NMR methods are described for the determination of four possible isomeric impurities in the novel anti-inflammatory agent benoxaprofen. The 2- and 3-chlorophenyl isomers were determined by GLC after alkaline hydrolysis and subsequent methylation. A rapid NMR procedure, using the lanthanide shift reagent tris- (1, 1, 1, 2, 2, 3, 3-heptafluoro- 7, 7-dimethyl- 4, 6-octanedionato)-europium, was developed for the 6- and 7- (alpha-methylacetic acid) isomers.

Neutrophil and rheumatoid factor-Immunoglobulin G insoluble complex interactions: phagocytosis and sequelae.

Studies utilizing 51CrCl3 labelled human immunoglobulin G have demonstrated a quantitative, time-related increase in the uptake of insoluble rheumatoid factor-immunoglobulin G complexes by human neutrophils. A burst of hexose monophosphate shunt activity occurs when these complexes are phagocytized by neutrophils as evidenced by the increased oxidation of glucose-l-14C to 14CO2. Metabolic and electron micrographic studies suggest that a heat stable serum factor is needed for maximum complex uptake and shunt activity.