Publications by authors named "S Listwak"
Article Synopsis
- Chronic social defeat (CSD) stress in mice leads to a significant accumulation of neutrophils in the meningeal area, while acute stress does not have the same effect.
- The study utilizes various techniques, like cytometry and single-cell sequencing, to show that CSD increases neutrophil movement from bone marrow through vascular channels.
- Blocking type I interferon (IFN-I) signaling can prevent negative behaviors linked to CSD stress, suggesting that targeting this pathway could help develop new treatments for stress-related disorders.
There is increasing interest in how immune cells, including those within the meninges at the blood-brain interface, influence brain function and mood disorders, but little data on humoral immunity in this context. Here, we show that in mice exposed to psychosocial stress, there is increased splenic B cell activation and secretion of the immunoregulatory cytokine interleukin (IL)-10. Meningeal B cells were prevalent in homeostasis but substantially decreased following stress, whereas Ly6C monocytes increased, and meningeal myeloid cells showed augmented expression of activation markers.
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- NF-κB is a key transcription factor that plays a major role in regulating immune responses and cell survival, and it can be found in neurons where it reacts to various stimuli.
- Measuring NF-κB activity in neurons is challenging due to the diverse cell types in the brain, so this study compared its activity between primary neocortical neurons, mixed brain cells, and liver cells using various assays.
- Results indicated that NF-κB activity in neurons is significantly lower than in other cell types, responding primarily to pro-inflammatory cytokines like TNFα and IL-β, while showing little reactivity to glutamate and other stimuli.
Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies.
J Neuroinflammation
October 2011
Background: The characterization and cellular localization of transcription factors like NF-κB requires the use of antibodies for western blots and immunohistochemistry. However, if target protein levels are low and the antibodies not well characterized, false positive data can result. In studies of NF-κB activity in the CNS, antibodies detecting NF-κB proteins have been used to support the finding that NF-κB is constitutively active in neurons, and activity levels are further increased by neurotoxic treatments, glutamate stimulation, or elevated synaptic activity.
View Article and Find Full Text PDFThe chemokine CCL27 has chemoattractant properties for memory T cells and has been implicated in skin allergic reactions. The present study reports the expression in the brain of two CCL27 splice variants localized in the cerebral cortex and limbic regions. CCL27-like immunoreactivity was identified mainly in neurons.
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