Production, purification and some properties of cholesterol oxidase from a Streptomyces Sp.

A Streptomyces Sp strain producing appreciable levels of cholesterol oxidase was selected. In the culture medium, cholesterol oxidase activity can reach 13,000 UI/l. Cholesterol oxidase was purified by means of centrifugation, tangential ultrafiltration, ion-exchange chromatography and gel filtration with a yield of 42% to a specific activity of 54 UI/mg.

Purification and characterization of a new fungal catalase.

We purified and characterized a new fungal catalase. The specific activity of the preparation obtained is 1500 UI/mg of protein. We found a molecular weight of 215,000 and a pI of 5.

A simplified method of production of choline oxidase suitable for choline assay.

A method of production of choline oxidase from Cylindrocarpon-didymum which is of sufficient purity to be used in the enzymatic assaying of choline has been described. The extraction and purification techniques used were ultrasonic disintegration, tangential ultrafiltration, and chromatography on DEAE-Sepharose Fast-Flow and on Sephacryl S 200 gel. The specific activity of the preparation obtained was 7.