Inter- and intraobserver variability in radiographic bone level assessment at Brånemark fixtures.

The aim was to determine inter- and intraobserver variability in radiographic bone level assessments at Brånemark fixtures and to study the influence of various factors (radiographic density; projection geometry; jaw in which the fixtures were inserted; degree of bone loss; time after fixture loading; and number of radiographs of each fixture) on the variability. Intraoral radiographs from bridge connection and 1- and 3-year check-ups from 15 upper and 15 lower jaws (172 fixtures) were assessed by 6 observers. Measurements were taken from a reference point on the fixture to the marginal bone level, and some were repeated by all observers after 1 months.

Betaine-homocysteine methyltransferase expression in porcine and human tissues and chromosomal localization of the human gene.

We have prepared antibodies against porcine liver betaine-homocysteine methyltransferase (BHMT; EC 2.1.1.

Identification of a gene that causes primary open angle glaucoma.

Glaucoma is a major cause of blindness and is characterized by progressive degeneration of the optic nerve and is usually associated with elevated intraocular pressure. Analyses of sequence tagged site (STS) content and haplotype sharing between families affected with chromosome 1q-linked open angle glaucoma (GLC1A) were used to prioritize candidate genes for mutation screening. A gene encoding a trabecular meshwork protein (TIGR) mapped to the narrowest disease interval by STS content and radiation hybrid mapping.

Fine mapping of the autosomal dominant juvenile open angle glaucoma (GLC1A) region and evaluation of candidate genes.

Juvenile Open Angle Glaucoma (GLC1A) is an autosomal optic neuropathy that has been localized previously to chromosome 1q. Here we report the fine mapping of the disease region using YACs and a high density of polymorphic microsatellite markers. This study utilized two large JOAG pedigrees genotyped at 36 loci from chromosome 1q21-q31 to refine the GLC1A locus to a approximately 3-cM region flanked by YAC-derived microsatellite markers D1S3665 and D1S3664.