Publications by authors named "S L Shafer"
Purpose: We conducted this study to assess cannabis use rates in the state of Kentucky relative to socioeconomic, demographic, and geographic factors, as well as reasons for use and modes of use, before the legal medical marijuana market commences in 2025.
Methods: We pooled Kentucky Behavioral Risk Factor Surveillance System (BRFSS) data for 2020-2021 and used weighted responses for all analyses. We estimated current cannabis use (at least once in the past 30 days), and heavy use (at least 20 of the past 30 days) prevalence rates for Appalachian, Delta, and Central geographic regions of Kentucky.
AMBRA1 controls the translation of immune-specific genes in T lymphocytes.
Proc Natl Acad Sci U S A
October 2024
T cell receptor (TCR) engagement causes a global cellular response that entrains signaling pathways, cell cycle regulation, and cell death. The molecular regulation of mRNA translation in these processes is poorly understood. Using a whole-genome CRISPR screen for regulators of CD95 (FAS/APO-1)-mediated T cell death, we identified AMBRA1, a protein previously studied for its roles in autophagy, E3 ubiquitin ligase activity, and cyclin regulation.
View Article and Find Full Text PDFBackground: Few studies have assessed the dose ratio of calcium gluconate to calcium chloride or defined the time course of change in serum ionized calcium concentration after intravenous injection.
Methods: In a bioequivalence (dose ratio) trial, parturients undergoing cesarean delivery were randomly assigned to receive calcium chloride (0.5 g) or calcium gluconate (1.
Article Synopsis
- - Kidney failure significantly impacts health, prompting a large-scale study of 406,504 participants to uncover genetic factors affecting kidney function, identifying 430 key genetic loci.
- - The research revealed that 56% of inherited differences in kidney function are linked to regulatory elements in kidney tubule epithelial cells, while 7% relate to podocyte cells, suggesting these are crucial for gene expression.
- - Further analysis using advanced techniques like enhancer assays and CRISPRi identified specific genes (NDRG1, CCNB1, and STC1) regulated by these genetic loci, shedding light on their roles in kidney function.
Article Synopsis
- Current estimates of genetic variants linked to Parkinson's disease (PD) show limitations and biases across different populations, complicating patient recruitment for clinical trials focused on genetic therapies.
- The Rostock Parkinson's disease (ROPAD) study analyzes data from 12,580 PD patients across 16 countries, revealing that 14.8% had a genetic test positive for PD-related variants, particularly in specific genes like GBA1 and LRRK2.
- Findings indicate higher positivity rates in patients with earlier onset (age ≤ 50) or a positive family history, emphasizing the need for more extensive genetic investigation to improve patient stratification for future clinical trials.