Radiation survival of two human cervical carcinoma cell lines after multifraction irradiation.

Purpose: Multifraction irradiation may contribute to radiation therapy treatment failure if selection of radiation resistant subpopulations occurs. We sought to determine whether surviving cells following daily fraction irradiation of two human cervical squamous cell carcinoma lines would express different radiation survival characteristics compared to the unirradiated parent.

Methods And Materials: A late-passage line (HTB35) and an early-passage line (RECA) received daily 2 Gy x-irradiation.

Augmentation by thyroxine of human granulosa cell gonadotrophin-induced steroidogenesis.

The objective of this study was to investigate the influence of thyroid hormone on gonadotrophin-induced oestradiol and progesterone secretion by human granulosa cells maintained in vitro. Granulosa cells were obtained by aspiration of pre-ovulatory follicles from women undergoing assisted reproductive technology. Ovulation induction was performed with gonadotrophin-releasing hormone agonist, human menopausal gonadotrophin and human chorionic gonadotrophin.

Influence of thyroxine on human granulosa cell steroidogenesis in vitro.

Purpose: The objective of this study was to investigate the influence of thyroid hormone on estradiol and progesterone secretion of human granulosa cells maintained in vitro.

Methods: Granulosa cells were obtained by aspiration of preovulatory follicles of woman undergoing assisted reproductive technology. Ovulation induction was performed with GnRH agonist, hMG, and hCG.

Thyroid hormones in human follicular fluid and thyroid hormone receptors in human granulosa cells.

Objective: To demonstrate the presence of thyroid hormone in human follicular fluid (FF) and the binding of antithyroid hormone antibodies in human granulosa cells (GCs).

Design: Follicular fluids and GCs collected from women undergoing oocyte retrieval after superovulation.

Setting: In Vitro Fertilization-America/Allegheny General Hospital and Reproductive Sciences Research Laboratories, the Department of Obstetrics and Gynecology, The Medical College of Pennsylvania/Allegheny Campus.