Development and standardization of a Loop-mediated isothermal amplification (LAMP) test for the detection of .

Bovine babesiosis is a tick-borne disease caused by protozoan parasites of the genus . is one of the most prevalent and economically important parasite species that infects cattle because of its impact on the meat and milk production industry. Effective disease control strategies should include detection of reservoir animals and early and specific pathogen detection using rapid, economical, sensitive, and specific detection techniques.

Llama and sheep ruminal fluid digestive capacity by in vitro gas production technique.

Background: The in vitro gas production technique has been used to evaluate forage fermentation kinetics. However, individual and animal species variation can change fermentation patterns due to differences in ruminal environment and microbiota. The aim was to verify whether rumen inoculum (RI) of llama had superior intrinsic digestion capacity and reduced methane (CH ) production compared to sheep RI using fescue and paspalum hay as substrates.

Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves.

It is known that nitrate inhibits ruminal methanogenesis, mainly through competition with hydrogenotrophic methanogens for available hydrogen (H) and also through toxic effects on the methanogens. However, there is limited knowledge about its effects on the others members of ruminal microbiota and their metabolites. In this study, we investigated the effects of dietary nitrate inclusion on enteric methane (CH) emission, temporal changes in ruminal microbiota, and fermentation in Holstein calves.

Ruminal effects of excessive dietary sulphur in feedlot cattle.

Sulphur (S) dietary excess can limit productive performance and increase polioencephalomalacia (PEM) incidence in feedlot cattle (FC). Sulphur excess ingested is transformed to hydrogen sulphide (H S) by sulfo-reducing ruminal bacteria (SRB), being high ruminal H S concentration responsible for aforementioned damages. As the ruminal mechanisms involved in H S concentrations increase have not been elucidated, this study aimed to evaluate the ruminal environment, and the association between ruminal H S and dissimilatory SRB (DSRB) concentration in FC experimentally subjected to S dietary excess.