Conformational Dynamics of Mitochondrial Inorganic Pyrophosphatase hPPA2 and Its Changes Caused by Pathogenic Mutations.

Inorganic pyrophosphatases, or PPases, are ubiquitous enzymes whose activity is necessary for a large number of biosynthetic reactions. The catalytic function of PPases is dependent on certain conformational changes that have been previously characterized based on the comparison of the crystal structures of various complexes. The current work describes the conformational dynamics of a structural model of human mitochondrial pyrophosphatase hPPA2 using molecular dynamics simulation, all-atom principal component analysis, and coarse-grained normal mode analysis.

The crystal structure of yeast mitochondrial type pyrophosphatase provides a model to study pathological mutations in its human ortholog.

Mutations in human ppa2 gene encoding mitochondrial inorganic pyrophosphatase (PPA2) result in the mitochondria malfunction in heart and brain and lead to early death. In comparison with its cytosolic counterpart, PPA2 of any species is a poorly characterized enzyme with a previously unknown 3D structure. We report here the crystal structure of PPA2 from yeast Ogataea parapolymorpha (OpPPA2), as well as its biochemical characterization.

Alteration of Structure and Characteristics of Concrete with Coconut Shell as a Substitution of a Part of Coarse Aggregate.

One of the most promising ways to solve the problem of reducing the rate of depletion of natural non-renewable components of concrete is their complete or partial replacement with renewable plant counterparts that are industrial and agricultural waste. The research significance of this article lies in the determination at the micro- and macro-levels of the principles of the relationship between the composition, the process of structure formation and the formation of properties of concrete based on coconut shells (CSs), as well as the substantiation at the micro- and macro-levels of the effectiveness of such a solution from the point of view of fundamental and applied materials science. The aim of this study was to solve the problem of substantiating the feasibility of concrete consisting of a mineral cement-sand matrix and aggregate in the form of crushed CS, as well as finding a rational combination of components and studying the structure and characteristics of concrete.

Constitutive inorganic pyrophosphatase as a reciprocal regulator of three inducible enzymes in Escherichia coli.

Background: Previous studies have demonstrated the formation of stable complexes between inorganic pyrophosphatase (PPase) and three other Escherichia coli enzymes - cupin-type phosphoglucose isomerase (cPGI), class I fructose-1,6-bisphosphate aldolase (FbaB) and l-glutamate decarboxylase (GadA).

Methods: Here, we determined by activity measurements how complex formation between these enzymes affects their activities and oligomeric structure.

Results: cPGI activity was modulated by all partner proteins, but none was reciprocally affected by cPGI.

Effect of Structure Variations in the Inter-subunit Contact Zone on the Activity and Allosteric Regulation of Inorganic Pyrophosphatase from Mycobacterium tuberculosis.

Hexameric inorganic pyrophosphatase from Mycobacterium tuberculosis (Mt-PPase) has a number of structural and functional features that distinguish it from homologous enzymes widely occurring in living organisms. In particular, it has unusual zones of inter-subunit contacts and lacks the N-terminal region common for other PPases. In this work, we constructed two mutant forms of the enzyme, Ec-Mt-PPase and R14Q-Mt-PPase.