In Vitro Investigation of the Antimicrobial Effect of Three Bisphosphonates Against Different Bacterial Strains.

Purpose: Since the first descriptions of medication-related osteonecrosis of the jaw (MRONJ) in 2003, the pathogenesis has remained unanswered. Recent histomorphometric studies have found several microorganisms, including Actinomyces, Bacillus, Fusobacterium, Staphylococcus, Streptococcus, Selenomonas, Treponema, and Candida albicans in necrotic bone. Polymerase chain reaction studies have recently confirmed the occurrence of 48 genera.

Genetic evidence of subaortic stenosis in the Newfoundland dog.

Subaortic stenosis (SAS) is a cardiac disorder with a narrowing of the descending aorta below the left ventricular outflow tract of the heart. It occurs in several species and breeds. The Newfoundland is one of the dog breeds where it is more common and usually leads to death at early adulthood.

Peptide-mediated reduction of silver ions on engineered biological scaffolds.

Herein we report the spontaneous reduction of silver ions into nanostructures by yeast surface-displayed glutamic acid (E(6)) and aspartic acid (D(6)) peptides. Light spectroscopy and electron microscopy reveal that silver ions are photoreduced in the presence of the polycarboxylic acid-containing peptides and ambient light, with an increase in reduction capability of E(6) expressing yeast over D(6) yeast. The importance of tethering peptides to a biological scaffold was inferred by observing the reduced particle forming capacity of soluble peptides with respect to corresponding yeast-displayed peptides.

Single M13 bacteriophage tethering and stretching.

The ability to present biomolecules on the highly organized structure of M13 filamentous bacteriophage is a unique advantage. Where previously this viral template was shown to direct the orientation and nucleation of nanocrystals and materials, here we apply it in the context of single-molecule (SM) biophysics. Genetically engineered constructs were used to display different reactive species at each of the filament ends and along the major capsid, and the resulting hetero-functional particles were shown to consistently tether microscopic beads in solution.

Virus-based toolkit for the directed synthesis of magnetic and semiconducting nanowires.

We report a virus-based scaffold for the synthesis of single-crystal ZnS, CdS, and freestanding chemically ordered CoPt and FePt nanowires, with the means of modifying substrate specificity through standard biological methods. Peptides (selected through an evolutionary screening process) that exhibit control of composition, size, and phase during nanoparticle nucleation have been expressed on the highly ordered filamentous capsid of the M13 bacteriophage. The incorporation of specific, nucleating peptides into the generic scaffold of the M13 coat structure provides a viable template for the directed synthesis of semiconducting and magnetic materials.