Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ-German Collection of Microorganisms and Cell Cultures decades ago. Genome-based phylogeny and DNA-DNA hybridization supported the assignment of these strains to 26 novel species and two novel subspecies and a reclassification of a species.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
August 2024
Two new strains JP48 and JP55 affiliated with the acidobacterial class have been isolated from fen soil sampled in the Fichtelgebirge Mountains near Bayreuth, Germany. Both strains were Gram-stain-negative, non-motile, non-spore-forming rods that divide by binary fission, segregate exopolysaccharide-like material and form capsules. Strains JP48 and JP55 grew at 4-36 °C (optimum at 27 °C), pH 3.
View Article and Find Full Text PDFMembers of the genus thrive in diverse habitats and use a broad range of recalcitrant organic molecules coupled to denitrification or O respiration. To gain a holistic understanding of the model organism EbN1, we studied its catabolic network dynamics in response to 3-(4-hydroxyphenyl)propanoate, phenylalanine, 3-hydroxybenzoate, benzoate, and acetate utilized under nitrate-reducing versus oxic conditions. Integrated multi-omics (transcriptome, proteome, and metabolome) covered most of the catabolic network (199 genes) and allowed for the refining of knowledge of the degradation modules studied.
View Article and Find Full Text PDFInterfacial water is a widespread lubricant down to the nanometer scale. We investigate the lubricities of molecularly thin HO and DO films confined between mica and graphene, via the relaxation of initially applied strain in graphene employing Raman spectroscopy. Surprisingly, the DO films are at least 1 order of magnitude more lubricant than HO films, despite the similar bulk viscosities of the two liquids.
View Article and Find Full Text PDFSilver iodide nanowires have been grown within tubular J-aggregates of the cyanine dye 3,3'-bis(2-sulfopropyl)-5,5',6,6'-tetrachloro-1,1'-dioctylbenzimida-carbo-cyanine (C8S3) from aqueous AgNO solutions. Crystal structure analysis by selected area electron diffraction (SAED), high resolution transmission electron microscopy (HRTEM) and energy-dispersive X-ray spectroscopy (EDXS) of single nanowires revealed that they are of silver iodide (AgI), while previously they were presumed to be of metallic silver. Iodine has not been added intentionally, but it is a remnant from the chemical synthesis of the dye and present in a dye:iodine ratio of almost 2:1, as revealed by inductively coupled plasma mass spectrometry (ICP-MS).
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