Publications by authors named "S Katzman"
Long noncoding RNAs are emerging as critical regulators of biological processes. While there are over 20,000 lncRNAs annotated in the human genome we do not know the function for the majority. Here we performed a high-throughput CRISPRi screen to identify those lncRNAs that are important in viability in human monocytes using the cell line THP1.
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- Sperm small RNAs, particularly tRNA fragments (tRFs), are influenced by environmental factors and play a role in passing down paternal traits.
- The study examines the impact of four specific ribonuclease genes (Rnase9-12) on sperm fertility and small RNA levels by creating knockout (KO) mice that lack these genes.
- KO male mice were found to be sterile, with notably low levels of tRFs in their sperm, revealing the importance of these genes in sperm development and function.
Article Synopsis
- * The study presents two key methods—RNA-seq and a high-throughput functional screen—to identify lncRNAs that play a role in monocyte-to-macrophage differentiation.
- * Four lncRNAs were found to potentially regulate this differentiation, with indications that they might work in conjunction with nearby protein-coding genes, though their exact roles and mechanisms are still unknown.
Article Synopsis
- * Researchers investigated the role of four paralogs of Angiogenin, which are expressed in the epididymis, by creating mice that lacked these genes; the results showed that these genes are critical for male fertility and RNA processing.
- * The KO mice were completely sterile, with their sperm unable to successfully fertilize oocytes due to impaired passage through the reproductive tract and a notable decrease in levels of tRFs, indicating these Angiogenin paralogs are vital for regulating sperm RNA composition.
Article Synopsis
- Sperm small RNAs, particularly tRFValCAC, play a crucial role in the epigenetic inheritance of environmental effects from fathers, but their functions are not fully understood.
- Researchers identified tRFValCAC as a prevalent small RNA in sperm that is enriched in the epididymis and delivered via extracellular vesicles.
- The study shows that tRFValCAC interacts with hnRNPAB to regulate its packaging into vesicles and is essential for mRNA processing and splicing in early embryos; inhibiting it disrupts splicing and delays embryonic development.