Computer modeling of three-dimensional reconstruction algorithm of cathodoluminescence material properties, analysis of errors, and optimization of variable parameters.

Three-dimensional (3-D) reconstruction of cathodoluminescence (CL) properties of materials (Saparin et al. 1997) is a new nondestructive technique that measures quantum yield information of subsurface layers as a set of two-dimensional image sections. This technique is able to synthesize a 3-D image without destroying the sample.

The morphology and composition of cholesterol, protein, and bilirubin deposits in dried human bile: cathodoluminescence and backscattered electron imaging.

This work is the first to deal with the application of color cathodoluminescence scanning electron microscopy (CCL SEM) and a novel version of combined imaging with backscattered electrons (CCL + BSE SEM) for the study of the composition of bile and its precipitation mechanisms. The present study demonstrates cholesterol, protein, and bilirubin distribution in deposits of normal and abnormal human bile after solution evaporation to full dryness. Qualitative CCL SEM analysis showed that dried bile remnants include different proportions of the above components.

Investigation of cholesterol, bilirubin, and protein distribution in human gallstones by color cathodoluminescence scanning electron microscopy and transmission electron microscopy.

The application of color cathodoluminescent scanning electron microscopy (CCL-SEM) for qualitative luminescence analysis of cholesterol, bilirubin, and protein in human gallastones was demonstrated. Images of these deposits (cholesterol, bilirubin, and protein) were formed in real colors (blue-cholesterol, red, orange-bilirubin, yellow, green-protein) in accordance with the cathodoluminescent spectrum for each control material. The other method described for transmission electron microscopy (TEM) of ultrathin sections provides more detailed characterization of the ultrastructure of cholesterol-containing regions and their spatial interrelations with bilirubin-containing regions.