Confirmation of parathion, methyl parathion and fenitrothion in biological material on thin-layer plates.

The present work describes a confirmatory procedure for the organophosphorus insecticides having a nitro group. These insecticides are reduced to corresponding amino derivatives on thin-layer plates, and the reduction product as well as the parent compound are resolved and identified by thin-layer chromatography.

Rapid method for extraction and colorimetric estimation of morphine in Indian opium.

A rapid method for the extraction and estimation of morphine in Indian opium is described. Methanolic extract of opium is treated with activated charcoal and the colorless extract obtained contains all of the major opium alkaloids. Morphine content is estimated colorimetrically by using hydrogen peroxide-copper-ammonia.

Effect of thiamine deficiency, pyrithiamine and oxythiamine on pyruvate metabolism in rat liver and brain in vivo.

Rats were fed either a thiamine-deficient diet of diets containing pyrithiamine or oxythiamine. When symptoms of thiamine deficiency appeared, the animals were injected intraperitoneally with [2-14C] pyruvate six to twelve minutes prior to sacrifice. Free glutamic and aspartic acids were isolated from liver and brain and degraded.