[Acetylcholine and effectiveness of oxidative phosphorylation in isolated rat hepatocytes].

It is shown that ADP and DNP does not intensify the respiration rate in hepatocytes of rats obtained by means of trypsin or EDTA. The same cells obtained using collagenase, phosphorylate added ADP and increase the respiration rate after DNP addition. Acetylcholine added to the cell suspension in a dose of 5 x 4 x 10(-8) M) increases the efficiency of oxidative phosphorylation.

[Acetylcholine activation of alpha-ketoglutarate oxidation in liver mitochondria].

Activation of alpha-ketoglutarate oxidation in the rat liver mitochondria takes place 15 and 30 min after intraperitoneal injection of acetyl choline. This mediator in doses of 25, 50 and 100 micrograms per 100 g of body weight causes a pronounced stimulation of phosphorylation respiration rate and calcium capacity of mitochondria with alpha-ketoglutarate oxidation. Acetyl choline is found to have a moderate inhibitory action on oxidation of lower (physiological) concentrations of succinate.