Use of an oversized AAV8 vector for CPS1 deficiency results in long-term survival and ammonia control.

Carbamoyl phosphate synthetase 1 (CPS1) deficiency, a urea-cycle disorder, results in hyperammonemia initiating a sequence of adverse events that can lead to coma and death if not treated rapidly. There is a high unmet need for an effective therapeutic for this disorder, especially in early neonatal patients where mortality is excessive. However, development of an adeno-associated virus (AAV)-based approach is hampered by large cDNA size and high protein requirement.

Nanophotonic platform for the highly sensitive detection of trypsin enzyme in human urine.

Trypsin is an important indicator of pancreatic disease. We introduce a nanophotonic test for the detection of trypsin. Our approach is based on the hydrolysis of β-casein by trypsin.

Efficacy of Different Combinations of Direct-Acting Antivirals Against Different Hepatitis C Virus-Infected Population Groups: An Experience in Tertiary Care Hospitals in West Bengal, India.

Hepatitis C virus (HCV) is a global public health problem, but advancements in HCV treatment have improved the cure rate. This study evaluated the effectiveness of direct-acting antivirals (DAAs) in HCV-infected patients from May 2021 to April 2023 in collaboration with tertiary care hospitals in West Bengal. The HCV viral load was monitored via qRT-PCR.

Biochar, zeolite, and ferric chloride effectively separate phosphorus and nitrogen (plus potassium) in swine manure: A coagulation-flocculation-sedimentation approach.

We studied the effectiveness of different coagulants (0.15 M FeCl, 0.40 M HCl, and 2.

Prevalence of use of nonpharmacological methods of pain relief among patients following onco surgeries - A prospective, observational cohort study from a single center.

Background And Aims: For the aggressive treatment of postoperative pain, nonpharmacological methods (NPMs) are gaining importance complementary to routine multimodal pain management. The primary aim of the study was to assess the incidence of use of NPMs in our hospital. Secondary objectives were to correlate the pain scores, patient satisfaction, and percentage of time the patient was in severe pain within 72 h postsurgery with the use of NPMs when in pain/not in pain.