Implementing precision cancer medicine in the public health services of Norway: the diagnostic infrastructure and a cost estimate.

Objective: Through the conduct of an individual-based intervention study, the main purpose of this project was to build and evaluate the required infrastructure that may enable routine practice of precision cancer medicine in the public health services of Norway, including modelling of costs.

Methods: An eligible patient had end-stage metastatic disease from a solid tumour. Metastatic tissue was analysed by DNA sequencing, using a 50-gene panel and a study-generated pipeline for analysis of sequence data, supplemented with fluorescence in situ hybridisation to cover relevant biomarkers.

Oxidative stress gradient in a medium during human corneal organ culture.

Purpose: Lipid peroxidation content was measured in an organ culture medium after one-week storage of human donor corneas. Moreover, the effects of the medium on oxidative stress, antioxidant capacity, and the proliferation of cultured human corneal cells were studied.

Methods: The medium was sampled from the upper and lower halves of storage vials and from controls (n=42).

Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan.

Purpose: Storage time for donor corneas in Optisol GS is limited compared to Eye Bank Organ Culture (EBOC). We here examine the epithelium on donor corneoscleral rims after primary storage in Optisol GS and subsequent incubation in EBOC.

Methods: Morphology was monitored by light and electron microscopy, expression of phenotypic and genotypic markers by immunohistochemistry and RT-PCR and changes in oxidative lipid and DNA damage by ELISA and COMET assay.

Oxidative stress in keratoconus?

Purpose: The purpose of this study was to establish the alterations of oxidative stress-related markers in keratoconus (KC) corneas.

Methods: A total of 6 healthy and 11 ectatic corneas (7 KC and 4 post-LASIK) were studied. Different oxidative stress-related markers were determined to assess their implication in the KC pathophysiology.

Intravitreal injection of bevacizumab induces inflammatory alterations in a uveitis experimental model.

Purpose: Bevacizumab is currently used as an intravitreal agent in the treatment of inflammatory-associated eye diseases. The aim of the current study is to explore the effects of the intravitreal injection of bevacizumab on aqueous humour cytokines and chemokines in an experimental uveitis model.

Methods: Endotoxin-induced uveitis was induced in rats by footpad injections.