Publications by authors named "S John Calise"
Regeneration of lost tissue requires biosynthesis of metabolites needed for cell proliferation and growth. Among these are the critical purine nucleotides ATP and GTP. The abundance and balance of these purines is regulated by inosine monophosphate dehydrogenase 2 (IMPDH2), which catalyzes the committing step of GTP synthesis.
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- Wooden house frames use simple geometric shapes for construction, while designing protein assemblies is more complex due to their irregular structures.
- This research introduces extendable protein building blocks that follow specific geometric standards, allowing for modular assembly that can be adjusted in size and shape.
- The team validates their protein nanomaterial designs through advanced imaging techniques, making it possible to construct large protein assemblies using straightforward architectural blueprints.
Inosine monophosphate dehydrogenase (IMPDH) is the rate-limiting enzyme in guanosine triphosphate (GTP) synthesis and assembles into filaments in cells, which desensitizes the enzyme to feedback inhibition and boosts nucleotide production. The vertebrate retina expresses two splice variants IMPDH1(546) and IMPDH1(595). In bovine retinas, residue S477 is preferentially phosphorylated in the dark, but the effects on IMPDH1 activity and regulation are unclear.
View Article and Find Full Text PDFInosine monophosphate dehydrogenase (IMPDH) is the rate-limiting enzyme in guanosine triphosphate (GTP) synthesis and is controlled by feedback inhibition and allosteric regulation. IMPDH assembles into micron-scale filaments in cells, which desensitizes the enzyme to feedback inhibition by GTP and boosts nucleotide production. The vertebrate retina expresses two tissue-specific splice variants IMPDH1(546) and IMPDH1(595).
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- The text discusses the construction of protein assemblies using extendable building blocks that follow specific geometric rules, similar to how a wooden house frame is built from regular lumber pieces.
- It highlights the development and validation of various protein designs, from simple shapes to complex nanostructures, using techniques like X-ray crystallography and electron microscopy.
- This approach allows for the deliberate assembly of large protein structures onto a 3D canvas, overcoming previous challenges related to the irregularity of protein shapes, and enables easier design of protein nanomaterials.