[Drug abuse of pregabalin - state of the situation, risks and means of control].

Pregabalin is an anticonvulsant widely used for the treatment of epilepsy and neuropathic pain. However, there is a growing concern about its misuse, particularly among drug users and patients with substance use disorders (SUD). It is often used in combination with other psychoactive molecules and at levels well above the recommended doses.

Application of isolated and strains as a probiotic starter culture during the industrial manufacture of Tunisian dry-fermented sausages.

For decades, lactic acid bacteria has been isolated and selected to be used as starter cultures in meat fermentation for standardization and management of quality of dry-fermented sausage which constitute a considerable challenge. The aim of this study was to evaluate the effect of strains, isolated from different origins, on qualities of dry-fermented sausages. These last, manufactured with different combinations of starter cultures ( + ), were ripened, using the same raw materials and conditions, for 45 days.

Freeze-dried, oven-dried, and microencapsulation of essential oil from as potential preservative agents of minced meat.

The present study was conducted to compare the antibacterial activity of oven-dried and freeze-dried along with its spray-dried microencapsulated essential oil in the preservation of minced beef meat. extracts were tested against mesophilic aerobic microorganisms, coagulase-positive , , sp., and the sulfite-reducing anaerobes.

Diversity and antimicrobial properties of lactic acid bacteria isolated from rhizosphere of olive trees and desert truffles of Tunisia.

A total of 119 lactic acid bacteria (LAB) were isolated, by culture-dependant method, from rhizosphere samples of olive trees and desert truffles and evaluated for different biotechnological properties. Using the variability of the intergenic spacer 16S-23S and 16S rRNA gene sequences, the isolates were identified as the genera Lactococcus, Pediococcus, Lactobacillus, Weissella, and Enterococcus. All the strains showed proteolytic activity with variable rates 42% were EPS producers, while only 10% showed the ability to grow in 9% NaCl.