[The purification and concentration of the rabies virus by a diafiltration concentration method].

Purified concentrates of rabies virus were prepared by both microfiltration chromatography and diafiltration methods. The diafiltration concentrate had a high level of protective activity and was not inferior to the microfiltration chromatographic one. The infectious activity was better retained after diafiltration, the chromatographic purity of both preparations was similar.

[The isolation and characteristics of hybridomas producing monoclonal antibodies to the structural proteins of the Vnukovo-32 strain of the rabies virus].

A series of hybridomas secreting monoclonal antibodies (MCA) to glycoprotein and nucleocapsid proteins of rabies virus strain Vnukovo-32 was selected as a result of fusion of splenocytes from immune BALB/c mice with cells of myeloma line Sp2/OAq14, screening and cloning by limiting dilution methods in semi-liquid agar. Four hybridomas secreted MCA to glycoprotein in high titres (5.0 x 10(5)-2.

[A comparison of 2 T-lymphoid cell lines persistently infected with the human immunodeficiency virus (HIV-1) with different productive capacities].

T-lymphoid cell lines (H9/CBL-4 and CEM/CBL-4) persistently infected with HIV-1 were observed simultaneously for 6.5 months. The virus activity was characterized by such parameters as the number of infected cells determined by fluorescent antibody technique, the total level of virus--specific protein synthesis determined by immune blotting method, and the capacity to infect H9 and CEM cells.

[Group- and type-specific antibodies to the gag-gene protein p26 of the human immunodeficiency virus immunological type 2 in infected patients].

The immunoreactivity of serum samples from HIV-2 infected persons was studied by radioimmunoprecipitation assay (RIPA) in homo- and heterotypic variants. In homotypic RIPA all sera studied have precipitated the viral glycoprotein with the high molecular weight, gp170. Some samples were active for gag-gene products, p57 and p26 in homotypic RIPA.

[A radioimmunoprecipitation method in the serodiagnosis of HIV infection: the development of the optimal conditions for its performance and the evaluation of the possibilities for its use].

The optimum conditions for using the method of radioimmunoprecipitation (RIP) for the detection of human immunodeficiency virus (HIV) in serum samples have been established. Out of several available cell lines persistently infected with HIV, specially selected line 17 has been chosen. The characteristic feature of this is the high and stable (under the conditions of prolonged cultivation) accumulation of virus-specific proteins in infected cells.