The search for proteins with immunochemical affinity to plant stress proteins at cold-adapted endemic Baikal fishes.

The search for proteins with immunochemical affinity to plant stress proteins in endemic Baikal fishes shows the presence of proteins, immunochemically related to plant heat-stabile proteins and plant uncoupling protein CSP 310. Western blotting showed that among the native cytoplasmic proteins of endemic Baikal fishes there are proteins immunochemically related to heat-stabile plant proteins with molecular weights about 480, 200-290, 150, 140 and about 90-100kD. SDS-electrophoresis showed the presence of polypeptides with molecular weights 23, 17 and 14kD in all species investigated and an additional 35kD polypeptide in Cottocomephorus grewingki.

Evidence for proteins specific for vascular elements in intact and cultured tissues and cells of maize.

A group of antigenically distinct proteins characteristic for the tissue complex of the vascular cylinders was found in maize (Zea mays L.) seedlings using an immunofiltration technique. Specific stelar antigens present in the fully developed stele (vascular cylinder) of the primary root were also found in steles extracted from adventitious roots and from the mesocotyl but were absent, within the limits of sensitivity of the immunodiffusion tests employed, in root cortex and epidermis.

Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.).

Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, in some cases for more than 2 years. These EA were distinct both from the meristematic antigens found in the intact seedlings and in callus cultures, and from organ-specific antigens found only in intact plants.

Identification of embryonal antigens of maize: Globulins as primary reserve proteins of the embryo.

Normal and γ-irradiated caryopses of Zea mays L. were germinated, and the degradation of embryonal antigens (EA) was followed in the endosperms, scutella and embryonic axes of the seedlings, using double immunodiffusion, immunoelectrophoresis and quantitative immunoprecipitation. The predominant transient EA were presumed to be storage proteins related to the reserve globulins of dicotyledonous seeds.

Embryonal antigens in maize caryopses: The temporal order of antigen accumulation during embryogenesis.

The sequential appearance of a specific group of embryonal antigens (EA), presumably globulins, was demonstrated in developing maize (Zea mays L.) caryopses using a double immunodiffusion test with absorption of common antigens. Cross immunoelectrophoresis was employed to follow the differential pattern of EA accumulation in the growing scutellum and embryonic axis.