Publications by authors named "S I Lok"
It is now possible to generate large volumes of high-quality images of biomolecules at near-atomic resolution and in near-native states using cryogenic electron microscopy/electron tomography (Cryo-EM/ET). However, the precise annotation of structures like filaments and membranes remains a major barrier towards applying these methods in high-throughput. To address this, we present TARDIS (ransformer-bsed apid imensionless nstance egmentation), a machine-learning framework for fast and accurate annotation of micrographs and tomograms.
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- The study investigates the role of the transcription factor Foxp2 in kidney fibrosis, a key process leading to chronic kidney disease (CKD) and kidney failure, highlighting its correlation with epithelial-to-mesenchymal transition (EMT).
- Analysis of human kidney biopsies and experiments in genetically modified mice demonstrate that increased Foxp2 expression is associated with various forms of CKD, and its deletion reduces inflammation and fibrosis in kidney tissue.
- Findings reveal that Foxp2 influences key cellular mechanisms, including the regulation of TGF-β signaling and cell cycle, ultimately affecting the progression of kidney fibrosis through the modulation of target genes related to cell growth and ECM production.
Article Synopsis
- The study investigates the relationship between surface characteristics like wetting and topography and their impact on ice adhesion strength (IAS), highlighting inconsistencies in previous research efforts.
- It summarizes various wetting and topography parameters linked to IAS and evaluates a broad range of surfaces using advanced measurement techniques.
- Findings reveal significant errors in past assumptions about IAS correlations, particularly challenging the notion of practical work of adhesion, and identify a promising wetting parameter that could effectively correlate with shear IAS on certain smooth surfaces.
Background: Obturator hernia is a rare type of pelvic hernia that often presents with vague symptoms that can easily be confused with other conditions. Delayed diagnosis can lead to increased morbidity and complications.
Case Presentation: A 67-year-old female with a history of rheumatoid arthritis presented with escalating right knee pain, unresponsive to increasing doses of opioids.
Structural studies on purified virus have revealed intricate architectures, but there is little structural information on how viruses interact with host cells . Cryo-focused ion beam (FIB) milling and cryo-electron tomography (cryo-ET) have emerged as revolutionary tools in structural biology to visualize the dynamic conformational of viral particles and their interactions with host factors within infected cells. Here, we review the state-of-the-art cryo-ET technique for viral structure studies and highlight exemplary studies that showcase the remarkable capabilities of cryo-ET in capturing the dynamic virus-host interaction, advancing our understanding of viral infection and pathogenesis.
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