Effect of enzyme-resistant starch on formation of 1,N(2)-propanodeoxyguanosine adducts of trans-4-hydroxy-2-nonenal and cell proliferation in the colonic mucosa of healthy volunteers.

The effect of enzyme-resistant starch (RS) on the development of colon cancer was reported to include both chemopreventive activity in humans and tumorigenic activity in animals. A study was performed to detect the influence of enzyme-RS on lipid peroxidation-induced DNA damage and cell proliferation. During two 4-week periods, 12 volunteers consumed a controlled diet in which starchy foods were enriched with a highly resistant amylomaize starch (Hylon VII) in the high-RS period and with an available corn starch in the low-RS period (second period).

Effects of resistant starch on the colon in healthy volunteers: possible implications for cancer prevention.

Recent evidence suggests that resistant starch (RS) is the single most important substrate for bacterial carbohydrate fermentation in the human colon. During two 4-wk periods. 12 healthy volunteers consumed a controlled basal diet enriched with either amylomaize starch (55.

In vitro fermentation of high-amylose cornstarch by a mixed population of colonic bacteria.

Background: Malabsorbed starch is probably the most important substrate for bacterial fermentation in the human large intestine. Fermentability of starch may depend on the composition of the colonic flora and its adaptation to the substrate supply.

Methods: Ten healthy volunteers were fed a controlled diet containing either 7.

The application of a semi-continuous colon simulation technique (Cositec) for studying the effects of clindamycin on microbial hindgut metabolism.

The semi-continuous colon simulation technique was used as an experimental in vitro model to study potential effects of clindamycin application on basic parameters of microbial hindgut metabolism. Hindgut contents from fistulated pigs kept on conventional diets were used as substrates for incubation. Measurements on reproducibility demonstrated the colon simulation technique as a suitable in vitro method to characterize microbial hindgut metabolism and those factors potentially influencing microbial fermentation.