Publications by authors named "S Hitoshi"
Neural stem cells (NSCs) are maintained in the adult mammalian brain throughout the animal's lifespan. NSCs in the subependymal zone infrequently divide and generate transit amplifying cells, which are destined to become olfactory bulb neurons. When transit amplifying cells are depleted, they are replenished by the quiescent NSC pool.
View Article and Find Full Text PDFNeural stem cells (NSCs) are maintained in the adult mammalian brain throughout the animal's lifespan. NSCs in the subependymal zone infrequently divide and generate transit amplifying cells, which are destined to become olfactory bulb neurons. When transit amplifying cells are depleted, they are replenished by the quiescent NSC pool.
View Article and Find Full Text PDFα1,3-Fucosyltransferase 9 (Fut9) is responsible for the synthesis of Lewis X [Le, Galβ1-4(Fucα1-3)GlcNAc] carbohydrate epitope, a marker for pluripotent or multipotent tissue-specific stem cells. Although Fut9-deficient mice show anxiety-related behaviors, structural and cellular abnormalities in the brain remain to be investigated. In this study, using in situ hybridization and immunohistochemical techniques in combination, we clarified the spatiotemporal expression of Fut9, together with Le, in the brain and retina.
View Article and Find Full Text PDFArticle Synopsis
- The CRISPR/Cas9 system, particularly the Streptococcus pyogenes Cas9 (WT-SpCas9), is important in gene therapy but limited by its requirement for a specific PAM sequence (NGG) to recognize target genes.
- Researchers developed an engineered Cas9 variant called SpCas9-NG, which can target broader PAM sequences (NGN), allowing for easier access to various gene mutations.
- In a study involving Huntington's disease, SpCas9-NG successfully repaired expanded CAG repeats in stem cells, demonstrating recovery of phenotypic issues in both neurons and derived animals, indicating its potential for targeting hard-to-reach mutations.