Comparative study of the effect of serum containing granulocyte-stimulating activity and colony-stimulating activity on hematopoiesis in triple-set diffusion chambers.

Serum obtained from rats with polyvinylpyrrolidone-induced sterile inflammation contains granulopoiesis-stimulating activity (GSA). In triple-set diffusion chambers (DC) with GSA in the middle chamber a stimulation of morphologically recognizable granulocytic cells (MRG) was found in mouse bone marrow cells cultivated in side chambers for 6 and 24 h. GSA also induced an increase in the number of CFU-GM when DC were inplanted into the peritoneum of normal rats.

A study of disturbances in granulocytopoiesis in humans using a diffusion chamber system.

Data are presented demonstrating that bone marrow granulocytic cells from normal persons continuously proliferate and mature during 5 d of cultivation in a diffusion chamber system (DC). Bone marrow granulocytopoiesis in 3 patients with different granulocytopoietic disorders was investigated using DC. In patient 1, with drug-induced agranulocytosis, bone marrow aspirated on the "day of recovery" revealed intensive proliferation in DC resulting in a highly significant increase of mature granulocytes.

Changes in stem cell compartments in CBA mice after treatment by serum obtained from leucophoretic rats.

Leucophoretic rat serum (LS) obtained from rats previously treated by i.p. injection of polyvinylpyrrolidone (PVP), specifically stimulates proliferation of morphologically recognizable granulocytic cells in vivo.

The simulation of bone marrow granulopoiesis of normal CBA mice in vitro and in vivo by serum obtained from leucophoretic rats.

The effect of leucophoretic serum (LS), obtained from rats with polyvinylpyrrolidone (PVP)-induced inflammation, on granulopoiesis in the bone marrow of normal CBA mice was studied. The following test systems were used: short term cultures (4 hr), diffusion chambers (8, 24, 48 and 72 hr) and in vivo assays (12, 24 and 48 hr). The results indicate that LS stimulates the proliferations of granulocytic cells by increasing the number of proliferative granulocytes in mitosis, as well as increasing the total number of proliferative granulocytes.

Effect of a stimulating factor on granulopoiesis in sublethally irradiated mice.

Sublethally irradiated mice were used as a model for an in vivo study of the effect of the stimulating factor present in rat leukophoretic serum (LS). LS was obtained from rats with inflammation induced by polyvinylpyrrolidone. Irradiated mice were injected i.