Vitamin K-dependent gamma-carboxyglutamic acid protein 1 promotes pancreatic ductal adenocarcinoma progression through stabilizing oncoprotein KRAS and tyrosine kinase receptor EGFR.

Background: Vitamin K-dependent γ-glutamic acid carboxylation (Gla) proteins are calcium-binding and membrane-associated, participating in coagulation, bone turnover, and cancer biology. The molecular function of transmembrane proline-rich Gla proteins (PRRGs) remains unexplored.

Methods: Analysis of pancreatic ductal adenocarcinoma (PDAC) datasets, including transcription profiles, clinical data, and tissue microarrays, was conducted to evaluate PRRG1 expression and its clinical relevance.

Grocery intervention and DNA-based assessment to improve diet quality in pediatric obesity: a pilot randomized controlled study.

Objective: We assessed the impact of a food-provisioning intervention on diet quality in children with obesity.

Methods: Participants (n = 33, aged 6-11 years) were randomly assigned to either usual care (intensive health behavior and lifestyle treatment) or intervention (usual care + food provisioning; high-fiber, low-dairy diet) for 4 weeks. The primary outcome was a change in child diet quality at Week 4.

dsDAP: An efficient method for high-abundance DNA-encoded library construction in mammalian cells.

DNA-encoded libraries are invaluable tools for high-throughput screening and functional genomics studies. However, constructing high-abundance libraries in mammalian cells remains challenging. Here, we present dsDNA-assembly-PCR (dsDAP), a novel Gibson-assembly-PCR strategy for creating DNA-encoded libraries, offering improved flexibility and efficiency over previous methods.

Intrinsic Solution to the Dilemma between High-Efficiency and External-Field-Free Spin-Orbit Torque Switching Based on Biaxial Devices.

Spin-orbit torque (SOT) is widely considered to be a fast and robust writing scheme for magnetic random-access memories (MRAMs). However, the requirements of field-free switching and high switching efficiency are often incompatible in SOT devices, placing a critical challenge on its improvement. Here we propose that by utilizing biaxial systems the dilemma between high-efficiency and external-field-free SOT switching can be solved intrinsically.