Immunocytochemical localization of human papilloma virus and cytomorphologic correlation in smears and biopsies of cervical flat condylomata.

The cytomorphologic features in cervical biopsies and smears associated with human papilloma virus antigen (Ag) expression as demonstrated by immunoperoxidase staining techniques are presented. There was good concordance between cytology and biopsy results with respect to immunoperoxidase staining for human papilloma virus Ag. Cytomorphologic scoring of low-grade lesions (cervical intraepithelial neoplasias, grades O and I) showed atypical mitoses, macronucleated koilocytes, superficial cell keratohyaline granules, and multinucleation to be more frequent in Ag+ biopsy specimens.

Cytodiagnosis of malignant lesions in cerebrospinal fluid. Review and cytohistologic correlation.

In an evaluation of the efficacy of cerebrospinal fluid (CSF) examination in our laboratory, 1,635 CSF specimens received between March 1, 1975, and December 31, 1982, were reviewed. All 111 positive samples (from 77 cases) and all 141 suspicious samples (from 83 cases) were reviewed microscopically, and the clinical records in the cases were rechecked. The 77 positive cases were confirmed by tissue and other studies: 23 as primary brain tumors, 26 as secondary carcinomas, 14 as leukemias, 12 as lymphomas and 2 as multiple myelomas.

Cytodiagnosis of endometrial malignant mixed mesodermal tumor.

The accuracy of endometrial aspiration smears obtained with the Isaacs cell sampler in the diagnosis of malignant mixed mesodermal tumors (MMMT) was compared to the results obtained with routine cervical and vaginal smears in five cases of MMMT found in a series of 220 endometrial aspirations. Cervical and vaginal smears previously taken on these patients were positive for adenocarcinoma or MMMT in two cases and suspicious for adenocarcinoma in the remaining three cases. Endometrial aspirates were positive for MMMT in three cases and positive for adenocarcinoma or MMMT in two cases.

Cytodiagnosis of lesions of the pancreas and related areas.

Our experience of six and one-half years with cyto-diagnosis of pancreatic and related lesions is presented. We studied 22 cases of direct (perioperative and endoscopic) aspiration (group A) and 14 cases of percutaneous aspiration using ultrasound guidance (group B). Cytohistologic correlation in the direct aspiration cases and cytoclinical correlation in the percutaneous aspiration cases showed a specificity of 90% in group A and 100% in group B.