Pathway Polygenic Risk Scores (pPRS) for the Analysis of Gene-environment Interaction.

Unlabelled: A polygenic risk score (PRS) is used to quantify the combined disease risk of many genetic variants. For complex human traits there is interest in determining whether the PRS modifies, i.e.

The Complex Relationship Between Cannabis Use and Mental Health: Considering the Influence of Cannabis Use Patterns and Individual Factors.

The relationship between cannabis use and mental health is complex, as studies often report seemingly contradictory findings regarding whether cannabis use results in more positive or negative treatment outcomes. With an increasing number of individuals using cannabis for both recreational (i.e.

Long-term persistence of glycemic dysregulation in patients with a history of pheochromocytoma/paraganglioma.

Context: Pheochromocytomas and paragangliomas (PPGLs) are rare endocrine tumors that frequently produce catecholamines. Catecholamine-induced cardiometabolic complications substantially contribute to increased morbidity and mortality in PPGL patients prior to surgical resection.

Objective: To determine whether markers of elevated cardiometabolic risk persist in patients following PPGL resection.

Chitosan obtained from black soldier fly larval cuticles expands the value chain and is effective as a biocontrol agent to combat plant pathogens.

The industrial use of certain insects, such as the black soldier fly (BSF, Hermetia illucens), has become a burgeoning way of converting residual biomass into a valuable source of biomolecules for the feed and food industry. Here, the integration of a valuable step as an upcycling technology using biological waste from the 5th instar BSF larvae processing as a source of bioactive chitosan was explored. The goal is to pave the way for sustainable chitosan production from insect-based resources in addition to proteins, lipids, and fertilizers.

4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts.

Multisubunit protein complexes are central to many cellular processes, and studying their activities and structures in vitro requires reconstitution via recombinant expression and purification. Obtaining targets at sufficient purity and scale typically involves screening several protein variants and expression hosts. Existing cloning strategies enable co-expression but are often time-consuming, labor-intensive, and host-specific, or involve error-prone steps.