Publications by authors named "S Grefte"
Objective: The peroxisome proliferator-activated receptor-alpha (PPARα) plays a central role in lipid metabolism in the liver by stimulating the expression of hundreds of genes. Accordingly, regulation by PPARα could be a screening tool to identify novel genes involved in hepatic lipid metabolism. Previously, the mitochondrial transporter SLC25A47 was suggested to play a role in energy metabolism and liver-specific uncoupling, but further research is lacking.
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- Dimeric nicotinamide nucleotide transhydrogenase (NNT) is a crucial enzyme located in the mitochondrial inner membrane, involved in converting NADP/NADH to NADPH/NAD while facilitating proton influx, but its specific roles and regulation in health and diseases like cancer are still not thoroughly understood.! -
- Research on NNT has been conducted through studies on gene mutations in specific models (like GCCD4 patients and C57BL/6J mice) and effects of NNT alterations in cancer cells, revealing both common and unique functional issues, yet information on NNT's physiological role in humans remains limited.! -
- To advance understanding of NNT's functions and effects in various conditions, future
Article Synopsis
- Mitochondrial morphology and membrane potential are key indicators of mitochondrial health, and these can be analyzed using fluorescent dyes in living cells.
- The study focuses on using TMRM and Mitotracker Green FM to assess both mitochondrial shape and membrane potential in primary human skin fibroblasts.
- An integrated protocol is provided for quantifying these parameters through epifluorescence microscopy, specifically highlighting its effectiveness with large, flat cells at high magnification.
Mitochondrial membrane potential (Δψ) and morphology are considered key readouts of mitochondrial functional state. This morphofunction can be studied using fluorescent dyes ("probes") like tetramethylrhodamine methyl ester (TMRM) and Mitotrackers (MTs). Although these dyes are broadly used, information comparing their performance in mitochondrial morphology quantification and Δψ-sensitivity in the same cell model is still scarce.
View Article and Find Full Text PDFSkeletal muscle relies on mitochondria for sustainable ATP production, which may be impacted by reduced oxygen availability (hypoxia). Compared with long-term hypoxia, the mechanistic in vivo response to acute hypoxia remains elusive. Therefore, we aimed to provide an integrated description of the Musculus gastrocnemius response to acute hypoxia.
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