, a Sentinel Species of Antibiotic Resistance in Cystic Fibrosis Respiratory Niche?

The importance and abundance of strict anaerobic bacteria in the respiratory microbiota of people with cystic fibrosis (PWCF) is now established through studies based on high-throughput sequencing or extended-culture methods. In CF respiratory niche, one of the most prevalent anaerobic genera is , and particularly the species The objective of this study was to evaluate the antibiotic susceptibility of this anaerobic species. Fifty isolates of cultured from sputum of 50 PWCF have been included.

Priming with intranasal lactobacilli prevents Pseudomonas aeruginosa acute pneumonia in mice.

Background: Increasing resistance to antibiotics of Pseudomonas aeruginosa leads to therapeutic deadlock and alternative therapies are needed. We aimed to evaluate the effects of Lactobacillus clinical isolates in vivo, through intranasal administration on a murine model of Pseudomonas aeruginosa pneumonia.

Results: We screened in vitro 50 pulmonary clinical isolates of Lactobacillus for their ability to decrease the synthesis of two QS dependent-virulence factors (elastase and pyocyanin) produced by Pseudomonas aeruginosa strain PAO1.

An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches.

Strict anaerobes are undeniably important residents of the cystic fibrosis (CF) lung but are still unknowns. The main objectives of this study were to describe anaerobic bacteria diversity in CF airway microbiota and to evaluate the association with lung function. An observational study was conducted during eight months.

Lactobacilli intra-tracheal administration protects from pulmonary infection in mice - a proof of concept.

The spreading of antibiotic resistance is a major public health issue, which requires alternative treatments to antibiotics. Lactobacilli have shown abilities to prevent pneumonia in clinical studies when given by oral route, certainly through the gut-lung axis involvement. Rationally, respiratory administration of lactobacilli has been developed and studied in murine model, to prevent from respiratory pathogens.

, a potential predictive biomarker of pulmonary infection in cystic fibrosis.

Introduction: pulmonary infections are the primary cause of morbi-mortality in patients with cystic fibrosis (CF). In this cohort study, the objective was to identify candidate biomarkers of infection within the airway microbiota.

Methods: A 3-year prospective multicentre study (PYOMUCO study) was conducted in Western France and included patients initially free for at least 1 year.