Publications by authors named "S Gofflo"
Article Synopsis
- - Skin tissue engineering is advancing rapidly due to clinical demands, the need to replace animal testing, and new technologies, with a focus on creating vascularized skin substitutes that can improve graft success rates.
- - Research emphasizes the importance of understanding the maturation and adaptability of capillary-like structures in these substitutes, particularly through a cell sheet approach that encourages angiogenesis while limiting endothelial cell density.
- - Findings reveal that adjusting VEGF levels and timing can influence the formation and coverage of these structures, highlighting the significance of a cell-derived microenvironment for mature, dynamic skin models suitable for various pharmacological studies.
Objective: Although the olfactory receptor OR2AT4 was described as involved in epidermal renewal, there is no data about a cosmetic active ingredient activating this receptor. The aim of this research work was thus to identify a natural molecule binding to this receptor in order to stimulate keratinocyte migration.
Methods: For this purpose, natural molecules were extracted from Cocos nucifera flour.
Objective: The early steps of HIV entry into intact vaginal mucosa still need to be clarified. Here we investigated how HIV translocated across the vaginal pluristratified epithelium, either by transcytosis or by uptake in Langerhans cells.
Methods: Using human primary fibroblasts and vaginal epithelial cells, we developed an in-vitro model of vaginal mucosa in which Langerhans cells could also be integrated.
Objective: Coinfection of rhesus macaques with human/simian immunodeficiency virus chimeras harbouring the minimal core-promoter/enhancer elements from HIV-1 clade B, C and E viral prototypes (STR-B, STR-C and STR-E) revealed a remarkable dichotomy in terms of spatio-temporal viral replication. The clade C chimera (STR-C) predominated in primary infection. The present study was aimed at identifying the origin of STR-C plasma viraemia at this infection phase.
View Article and Find Full Text PDFAlthough the primary determinant of cell tropism is the interaction of viral envelope or capsid proteins with cellular receptors, other viral elements can strongly modulate viral replication. While the HIV-1 promoter is polymorphic for a variety of transcription factor binding sites, the impact of these polymorphisms on viral replication in vivo is not known. To address this issue, we engineered isogenic SIVmac239 chimeras harboring the core promoter/enhancer from HIV-1 clades B, C, and E.
View Article and Find Full Text PDF