Protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker and Th1 cytokine detection.

Flow cytometry characterization of antigen-specific polyfunctional T cells is a valuable tool to study adaptive immunity. Here, we present a protocol for flow cytometry immunophenotyping of human antigen-specific T cells by activation-induced marker (AIM) and Th1 cytokine detection. We describe steps for preparing peripheral blood mononuclear cells (PBMCs) for stimulation followed by washing and staining PBMCs for flow cytometry.

Perioperative Vision Loss Following Prone Spine Surgery: A Case-Control Study.

Purpose: Perioperative vision loss (POVL) is a rare and devastating complication following prone spine surgery. Due to the rare nature of this complication, there is limited research available about patient and surgical risk factors that increase the risk of POVL. The objective of this study was to investigate associated risk factors for POVL with use of the National Surgical Quality Improvement Program (NSQIP) database.

Patient-specific Predictors of Surgical Delay in a Large Tertiary-care Hospital Operating Room.

Purpose: The purpose of this study was to describe patient-specific factors predictive of surgical delay in elective surgical cases.

Design: Retrospective cohort study.

Methods: Data were extracted retrospectively from the electronic health record of 32,818 patients who underwent surgery at a large academic hospital in Los Angeles between May 2012 and April 2017.

California Certified Registered Nurse Anesthetist Workforce Study.

Background: Nurse anesthesia in California has been instrumental in shaping Certified Registered Nurse Anesthetist (CRNA) practice nationally, but to date, no workforce data has been published on this group of Advanced Practice Registered Nurses.

Purpose: The purpose of this workforce study was to explore the demographic information, education, and practice patterns of CRNAs working in California.

Methods: Survey methodology was conducted to gather workforce data from a sample of licensed CRNAs working in California.

Building and environmental acoustics in obsolete residential neighbourhoods: The case of San Pablo, Spain.

In order to establish an agreement between the objectives of acoustic quality in the outdoor environment and the acoustic insulation in a building as required in the current legislation in Spain, the acoustic insulation of typical social housing has been studied in the residential neighbourhood of San Pablo in Seville. Field measurements based on acoustic sonometry have been performed in its public areas, together with the consultations to the strategic noise maps prepared by the administration of the city and with data from a questionnaire answered by neighbours on the perception of environmental and domestic noise. These inputs have enabled calculations of airborne and impact noise insulation in a typical dwelling of the neighbourhood.