Roughness statistical influence on cell adhesion using profilometry and multiscale analysis.

In this study, two series of 11 samples of TiAl6V4 titanium alloy and 316L stainless steel have been polished in an isotropic manner at different levels in order to quantify the influence of biomaterial roughness on cell behavior. Topography was measured by a tactile profilometer and a multiscale analysis has been carried out. Human osteoblasts have been cultured on those samples.

Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography.

Our objective in this study was to determine whether a threshold in sensitivity of human mesenchymal stem cells (hMSC) to isotropic roughness exists. Using electrical discharge machining a very wide range of roughnesses (1.2μm View Article and Find Full Text PDF

Statistical approach of chemistry and topography effect on human osteoblast adhesion.

Our objective in this work was to determine statistically the relative influence of surface topography and surface chemistry of metallic substrates on long-term adhesion of human bone cell quantified by the adhesion power (AP). Pure titanium, titanium alloy, and stainless steel substrates were processed with electro-erosion, sandblasting, or polishing giving various morphologies and amplitudes. The surface chemistry was characterized by X-ray photoelectron spectroscopy (XPS) associated with an extensive analysis of surface topography.