Publications by authors named "S Gilhaus"
Disruption of the actin cytoskeleton in AR4-2J pancreatic acinar cells led to an increase in cytosolic protein tyrosine phosphatase activity, abolished bombesin-induced tyrosine phosphorylation and reduced bombesin-induced amylase secretion by about 45%. Furthermore, both tyrosine phosphorylation and amylase secretion induced by phorbol ester-induced activation of protein kinase C were abolished. An increase in the cytosolic free Ca2+ concentration by the Ca2+ ionophore A23187 had no effect on tyrosine phosphorylation but induced amylase release.
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- The study examines how protein tyrosine phosphorylation influences amylase secretion in AR4-2J cells, noting that various secretagogues, including bombesin and PMA, promote this phosphorylation.
- Both PMA and the phosphatase inhibitor pervanadate enhance amylase secretion similarly, and their effects can be blocked by specific tyrosine kinase inhibitors.
- The research concludes that while protein kinase C activation kicks off a cascade leading to secretion, additional calcium-dependent processes happen later, highlighting a multi-step mechanism in amylase release regulation.