Decoding Gene Networks Controlling Hypothalamic and Prethalamic Neuron Development.

Neuronal subtypes derived from the embryonic hypothalamus and prethalamus regulate many essential physiological processes, yet the gene regulatory networks controlling their development remain poorly understood. Using single-cell RNA- and ATAC-sequencing, we analyzed mouse hypothalamic and prethalamic development from embryonic day 11 to postnatal day 8, profiling 660,000 cells in total. This identified key transcriptional and chromatin dynamics driving regionalization, neurogenesis, and differentiation.

Polycystic ovary syndrome and risk of differentiated thyroid cancer: A nationwide, register-based cohort study based on Danish health data.

Objectives: To evaluate the association between polycystic ovary syndrome (PCOS) and the risk of differentiated and papillary thyroid cancer.

Methods: We included all individuals assigned female at birth and born during 1962-1996 in Denmark. Information on vital status, PCOS- and cancer diagnoses, and covariates were attained from various Danish nationwide registers.

Camera-on-tip endoscope for cardiovascular diagnostics and surgical guidance.

Cardiovascular imaging with camera-on-tip endoscopes has the potential to provide physiologically relevant data on the tissue state and device placement that can improve clinical outcomes. In this work, we review the unmet clinical need for image-based cardiovascular diagnostics and guidance for minimally invasive procedures. We present a 7 Fr camera-on-tip endoscope with fibre-coupled multispectral illumination that includes methods for imaging in a blood-filled field of view (FOV).

'You would rather not fill your body with pills'-patient perspectives on polypharmacy and medication reviews by pharmacists in general practice.

Introduction: Polypharmacy is widespread. The demographic shift toward older patients receiving multiple medications increases risk and drug-related problems in these patients.

Objective: To investigate patient perspectives on polypharmacy and the experienced effects of medication reviews by pharmacists in general practice.

Genome Insights into Beneficial Microbial Strains Composing SIMBA Microbial Consortia Applied as Biofertilizers for Maize, Wheat and Tomato.

For the safe use of microbiome-based solutions in agriculture, the genome sequencing of strains composing the inoculum is mandatory to avoid the spread of virulence and multidrug resistance genes carried by them through horizontal gene transfer to other bacteria in the environment. Moreover, the annotated genomes can enable the design of specific primers to trace the inoculum into the soil and provide insights into the molecular and genetic mechanisms of plant growth promotion and biocontrol activity. In the present work, the genome sequences of some members of beneficial microbial consortia that have previously been tested in greenhouse and field trials as promising biofertilizers for maize, tomato and wheat crops have been determined.