Large-Scale Production of Isocitric Acid Using Yeast with Further Down-Stream Purification.

Isocitric acid (ICA) refers to a group of promising regulators of energy metabolism which has antistress, antihypoxic, and antioxidant activities. In this paper, we reported a process of ICA production from rapeseed oil using yeast VKM Y-2373 in a 500-L fermentor. The producer synthesized 64.

Effect of Nitrogen Concentration on the Biosynthesis of Citric Acid, Protein, and Lipids in the Yeast .

yeast is well known to be able to synthesize citric acid (CA) in large amounts. This study deals with CA biosynthesis, the production of biomass, as well as the accumulation and composition of proteins and lipids in VKM Y-2373 grown in media with glucose at different concentrations of ammonium sulfate (from 2 to 10 g/L). It was found that these concentrations of nitrogen source are limiting for the growth of and that nitrogen deficiency is the main cause of CA excretion.

Optimization of medium composition and fermentation conditions for α-ketoglutaric acid production from biodiesel waste by Yarrowia lipolytica.

This work demonstrates the ability of the yeast Yarrowia lipolytica cultivated on biodiesel waste to synthesize α-ketoglutaric acid with a minimal content of pyruvic acid as the main byproduct. The key factor promoting the microbial production of α-ketoglutaric acid from the waste is a strong deficiency of thiamine in the cultivation medium. The production of α-ketoglutaric acid by the yeast can be regulated by changing the concentration of nitrogen, iron, zinc, copper, and manganese in the medium, as well as by pH medium and the aeration rate.

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory.

Within this work, the microbial synthesis of (2,3)-isocitric acid (ICA), a metabolite of the nonconventional yeast , from biodiesel waste, has been studied. The selected strain VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions.

Microbial production of (2R,3S)-isocitric acid: state of the arts and prospects.

(2R,3S)-isocitric acid has long been used only as a specific biochemical reagent. However, there is ever increasing evidence that it can also be used as an original promising substance for prevention and treatment of some diseases. The review considers the results of longtime research in our laboratory and the data of other researchers related to microbial synthesis of (2R,3S)-isocitric acid, derivation and selection of active microbial producers, development of their cultivation conditions, as well as the results of study of the mechanism of acid overproduction, and regulation of enzymes involed in this process.