Resistive-Pulse Sensing Coupled with Fluorescence Lifetime Imaging Microscopy for Differentiation of Individual Liposomes.

Characterization of individual biological nanoparticles can be significantly improved by coupling complementary analytical methods. Here, we combine resistive-pulse sensing (RPS) with fluorescence lifetime imaging microscopy (FLIM) to differentiate liposomes at the single-particle level. RPS measures the particle volume, shape, and surface-charge density, and FLIM determines the fluorescence lifetime of the fluorophore associated with the lipid membrane.

Describing a Transition to Adult Type 1 Diabetes Care Model at the McGill University Health Centre.

Aims: To describe a type 1 diabetes melllitus (T1DM) transition care model by assessing clinic attendance, glycemic management, and diabetes-related hospitalizations.

Methods: This is a descriptive longitudinal single-center study of patients with T1DM aged 18 to 25 referred to our transition clinic from 2012 to 2021 (N=179).

Results: Our data analysis demonstrates an average clinic attendance rate of 79% and mean time between last pediatric and first adult visit of 6.

Quantification of subcellular RNA localization through direct detection of RNA oxidation.

Across cell types and organisms, thousands of RNAs display asymmetric subcellular distributions. The study of this process often requires quantifying abundances of specific RNAs at precise subcellular locations. To analyze subcellular transcriptomes, multiple proximity-based techniques have been developed in which RNAs near a localized bait protein are specifically labeled, facilitating their biotinylation and purification.