Role of endothelial dysfunction and lymphocyte-platelet adhesion in the pathogenesis of bile peritonitis.

Bile peritonitis is associated with endotoxemia, endothelial dysfunction, and increased level of pro-inflammatory cytokines. Under these conditions, serum concentration of NO metabolites (nitrate/nitrite ratio) and NO production by platelets significantly increase in animals with bile peritonitis, which reflects the development of inflammatory process and its antithrombotic activity, respectively. At the same time, platelet adhesion to lymphocytes increased in animals with bile peritonitis.

[The correction of the endothelial dysfunction by the experimental biliary peritonitis].

The purpose of the study is to increase the effectiveness of treatment of biliary peritonitis. The studies strongly suggest that in the early stages of the animals biliary peritonitis development against the background of endogenous intoxication in the body occur metabolic and functional disorders. The reason of this disorders is endothelial dysfunction with the violation of synthesis of such biologically active substances as endothelin-1, NO, von Willebrand factor and cytokines in the blood plasma.

[Phagocytic activity of neutrophils in conditions of experimental choleperitonitis].

In experiments on animals with choleperitonitis were signs of systemic inflammatory response syndrome, "rejuvenation" ofwbc as through the mobilization of neutrophils membrane pool and due to the activation of bone marrow reserve of granulocytes in response to the development of endogenous intoxication. The analysis of the results of the study also showed that animals with choleperitonitis have the change of the neutrophils functional state, which reflected in changes of their cytochemical profile, accompanied by a decrease in total activity and functional reserve of neutrophils, inhibition of nonspecific link of the immune system, manifested by a decrease of the neutrophils number, which are able to absorb and digest microbal cells.