3D structure reconstruction of nanoengineered polymeric capsules using Coherent X-Ray diffraction imaging.

Nanoengineered polymeric capsules (NPCs) are smart objects that can be filled in with some desired chemical substance. They are considered among the most versatile tools in biology, pharmacy, medicine etc. Most often they have been used as containers for drug delivery.

Synthesis and crystal structures of nickel(ii) and palladium(ii) complexes with o-carboranyl amidine ligands.

A number of new nido-carboranyl amidines 10-R(CH)NHC(Et)[double bond, length as m-dash]HN-7,8-CBH (n = 2, R = OH, OMe, and NMe; n = 3, R = OH) were synthesized by the nucleophilic addition of amino alcohols and N,N-dimethylethylenediamine to the highly activated -C[triple bond, length as m-dash]N- triple bond of the 10-propionitrilium derivative of nido-carborane. A similar reaction of 10-EtC[triple bond, length as m-dash]N-7,8-CBH with ethylenediamine unexpectedly resulted in the cleavage of the C[triple bond, length as m-dash]N bond to form the ammonium derivative 10-HN-7,8-CBH. The complexation of the synthesized carboranyl amidines 10-MeO(CH)NHC(Et)[double bond, length as m-dash]HN-7,8-CBH and 10-MeN(CH)NHC(Et)[double bond, length as m-dash]HN-7,8-CBH with nickel and palladium phosphine complexes [(PhP)MCl] (M = Ni, Pd) was studied.

Surface NKG2C Identifies Differentiated αβT-Cell Clones Expanded in Peripheral Blood.

T cells that express CD56 in peripheral blood of healthy humans represent a heterogeneous and poorly studied subset. In this work, we analyzed this subset for NKG2C expression. In both CD56 and CD56 subsets most of the NKG2C T cells had a phenotype of highly differentiated CD8 TEMRA cells.

CD56 CD57 NKG2C NK cells retaining proliferative potential are possible precursors of CD57 NKG2C memory-like NK cells.

Formation of the adaptive-like NK cell subset in response to HCMV infection is associated with epigenetic rearrangements, accompanied by multiple changes in the protein expression. This includes a decrease in the expression level of the adapter chain FcεRIγ, NKp30, and NKG2A receptors and an increase in the expression of NKG2C receptor, some KIR family receptors, and co-stimulating molecule CD2. Besides, adaptive-like NK cells are characterized by surface expression of CD57, a marker of highly differentiated cells.

HLA-DR-expressing NK cells: Effective killers suspected for antigen presentation.

HLA-DR-expressing cells comprise an intriguing group of NK cells, which combine phenotypic characteristics of both NK cells and dendritic cells. These cells can be found in humans and mice; they are present in blood and tissues in healthy conditions and can expand in a spectrum of pathologies. HLA-DR NK cells are functionally active: they produce proinflammatory cytokines, degranulate, and easily proliferate in response to stimuli.