The Complementary Effect of Cholesterol and Vitamin E Preloaded in Cyclodextrins on Frozen Bovine Semen: Motility Parameters, Membrane Integrity and Lipid Peroxidation.

Background: During cryopreservation sperm cells suffer from two major deleterious impacts: oxidative stress and cold shock.

Objective: To investigate in bovine species the benefit of cholesterol and vitamin E, both loaded in cyclodextrins, as a double protection against oxidative stress and cold shock.

Materials And Methods: Semen was collected from nine mature bulls using an artificial vagina and each ejaculate was split into four equal aliquots.