Publications by authors named "S Eghbalsaied"
Base editors are a type of double-stranded break (DSB)-free gene editing technology that has opened up new possibilities for precise manipulation of mitochondrial DNA (mtDNA). This includes cytosine and adenosine base editors and more recently guanosine base editors. Because of having low off-target and indel rates, there is a growing interest in developing and evolving this research field.
View Article and Find Full Text PDFOn-target integration of large cassettes via homology-directed repair (HDR) has several applications. However, the HDR-mediated targeted knock-in suffered from low efficiency. In this study, we made several large plasmids (12.
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- Female domestic goats often struggle to nurse multiple kids due to having a limited number of functional teats, making teat functionality crucial for breeding and kid health.
- A study analyzed data from 697 goats across various breeds in Iran to examine traits like litter size and mammary gland histology, revealing that certain breeds (like MOHA, BORE, and SANN) had larger litter sizes.
- Results indicated that the gene FGF-2 was expressed differently in goats based on the number of functional teats, suggesting potential areas for further research on teat variations and their impacts on breeding outcomes.*
An Electrochemical Protocol for CRISPR-Mediated Gene-Editing of Sheep Embryonic Fibroblast Cells.
Cells Tissues Organs
April 2023
Genetic engineering of farm animals is commonly carried out via cell-mediated transfection followed by somatic cell nuclear transfer. However, efficient transfer of exogenous DNA into ovine embryonic fibroblast (EF) cells without compromising cell viability has remained a challenging issue. Here, we aimed to develop a protocol for electrotransfection of sheep EF cells.
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