Generation of super-resolution images from barcode-based spatial transcriptomics by deep image prior.

Spatially resolved transcriptomics (ST) has revolutionized the field of biology by providing a powerful tool for analyzing gene expression in situ. However, current ST methods, particularly barcode-based methods, have limitations in reconstructing high-resolution images from barcodes sparsely distributed in slides. Here, we present SuperST, an algorithm that enables the reconstruction of dense matrices (higher-resolution and non-zero-inflated matrices) from low-resolution ST libraries.

A highly prevalent and specific cryptic plasmid pBI143 for human fecal pollution tracking in a subtropical urban river.

Microbial source tracking (MST) is a critical tool for identifying sources of human and animal fecal pollution in aquatic environments. To enhance human fecal pollution tracking, this study evaluated the performance characteristics of pBI143, a cryptic plasmid recently identified for potential MST applications. Nucleic acid samples from ten animal species were screened for pBI143, revealing its presence in a small number of pigs, cows, dogs, cats, and flying fox fecal samples.

Evaluation of rest interval following a series of tuck jumps on anaerobic performance.

Background/objective: This study assessed the influence of rest interval duration after tuck jumps on 10-s Wingate outcomes and countermovement jump height.

Methods: Eighteen resistance trained individuals (males: n = 10, 21.3 ± 3.