Evolution of Tribolium madens (Insecta, Coleoptera) satellite DNA through DNA inversion and insertion.

Two different satellite DNAs from tenebrionid species Tribolium madens (Insecta, Coleoptera) have been detected, cloned, and sequenced. Satellite I comprises 30% of the genome; it has a monomer size of 225 bp and a high A + T content of 74%. Satellite II, with a monomer size of 711 bp and A + T content of 70%, is less abundant, making 4% of the total DNA.

Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli.

Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp.