Publications by authors named "S Diaz-Sanchez"
The α-Gal syndrome (AGS) is an IgE-mediated tick borne-allergy that results in delayed anaphylaxis to the consumption of mammalian meat and products containing α-Gal. Considering that α-Gal-containing microbiota modulates natural antibody production to this glycan, this study aimed to evaluate the influence on tick salivary compounds on the gut microbiota composition in the zebrafish (Danio rerio) animal model. Sequencing of 16 S rDNA was performed in a total of 75 zebrafish intestine samples, representing different treatment groups: PBS control, Ixodes ricinus tick saliva, tick saliva non-protein fraction (NPF), tick saliva protein fraction (PF), and tick saliva protein fractions 1-5 with NPF (F1-5).
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- - The study investigates genetic variability in specific mitochondrial and nuclear genes of the cattle tick (*Rhipicephalus (Boophilus) microplus*) collected from the Neotropics and compared with sequences from GenBank, focusing on identifying clades and the impact of climate on genetic divergence.
- - Findings reveal that only one clade (clade A) appears in the Nearctic-Neotropics, with low genetic diversity and divergence rates in Neotropical populations, suggesting inadequate correlation with geographic distances or environmental factors.
- - The low gene variability may stem from recent tick introductions, effective population exchanges, and minimal climate impact on gene evolution, which is significant for understanding ecological dynamics and controlling cattle tick infestations.
Background: Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to unravel the molecular and immune mechanisms underlying this syndrome. The objective of this study is the characterization of tick salivary components and proteins with and without α-Gal modifications involved in modulating human immune response against this carbohydrate.
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- Researchers are exploring the use of tick microbiota to combat tick-borne diseases like human granulocytic anaplasmosis (HGA) by leveraging a method called Frankenbacteriosis. !* -
- Frankenbacteriosis involves genetically modifying symbiotic bacteria in ticks to mimic pathogens, creating competition that reduces the opportunity for infection. !* -
- The modified bacteria produce a surface protein, MSP4, which interacts with the tick’s infection receptors, potentially allowing for lab-grown, genetically modified ticks that lower the transmission of pathogens and reduce HGA disease risks in the field. !*
Background: Excessive and irrational use of antibiotics as growth promoters in poultry has been one of key factors contributing to increased emergence of antibiotics resistant bacteria. Several alternatives for antibiotic growth promoters are being sought, and the search for effective probiotics to be used as feed additives is amongst the promising ones. Our study aimed to isolate and test potential probiotics bacteria from cloacal swabs of various indigenous chicken (Gallus domesticus) breeds from rural outskirts of the Kathmandu valley (Nepal).
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