Publications by authors named "S D Savkovic"
Study Question: What is the natural history of elective autologous sperm cryostorage prior to gonadotoxic treatment?
Summary Answer: We estimate large sample median times to transfer for use, to the man's death or to discard of sperm, and their determinants, as the key operational outcomes of sperm cryostorage.
What Is Known Already: No large sample studies of the natural history of sperm cryostorage prior to gonadotoxic treatment are reported.
Study Design, Size, Duration: This observational single-centre study covered 45 years of outcomes with a survival analysis for sperm cryostorage prior to scheduled gonadotoxic treatment, and its determinants.
Background & Aims: The obesity epidemic is associated with increased colon cancer progression. As lipid droplets (LDs) fuel tumor growth, we aimed to determine the significance of diacyltransferases (diacylglycerol o-acyltransferases 1 and 2 [DGAT1/2]), responsible for LDs biogenesis, in obesity-mediated colonic tumorigenesis.
Methods: Human colon cancer samples, colon cancer cells, colonospheres, and Apc colon cancer mouse model on a high-fat diet were employed.
Context: Endogenous and exogenous androgens increase circulating erythrocytes and hemoglobin but their effects on erythrocyte lifespan is not known.
Objective: To investigate androgen effects on immature and mature erythrocyte lifespan in humans and mice using novel nonradioactive minimally invasive methods.
Design: Human erythrocyte lifespan was estimated using alveolar carbon monoxide concentration and blood hemoglobin in Levitt's formula in hypogonadal or transgender men before and up to 18 weeks after commencing testosterone (T) treatment.
Article Synopsis
- Desmoplastic small round cell tumors (DSRCT) are aggressive pediatric sarcomas linked to the EWSR1::WT1 fusion oncogene, with poor survival rates of 15% to 25% due to ineffective standard treatments.
- The study demonstrated that targeting EWSR1::WT1 is crucial for DSRCT growth and identified its unique role in activating genes related to the cyclin D-CDK4/6-RB pathway.
- Treatment with the FDA-approved CDK4/6 inhibitor palbociclib effectively reduced DSRCT growth in models, suggesting a promising new therapeutic option for this challenging cancer.
Dried blood spot sampling of testosterone microdosing in healthy females.
J Steroid Biochem Mol Biol
June 2024
Capillary dried blood spot (DBS) analysis coupled with multi-analyte steroid liquid chromatography mass spectrometry (LCMS) is attractive for field studies, home-based self-sampling as well as clinical trials by eliminating costly and laborious sample processing involving venipuncture and frozen storage/shipping while providing multiple steroid measurements from a single small sample. We investigated steroid measurements in DBS samples stored for four years at room temperature prior to analysis compared with the original venipuncture serum samples. Healthy women (n=12) provided paired DBS and blood samples over two weeks run-in before seven days treatment with daily transdermal T gel (12.
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