Publications by authors named "S D Oreshkova"
Background: Development of a universal vaccine capable to induce antibody responses against a broad range of influenza virus strains attracts growing attention. Hemagglutinin stem and the exposed fragment of influenza virus M2 protein are promising targets for induction of cross-protective humoral and cell-mediated response, since they contain conservative epitopes capable to induce antibodies and cytotoxic T lymphocytes (CTLs) to a wide range of influenza virus subtypes.
Methods: In this study, we generated DNA vaccine constructs encoding artificial antigens AgH1, AgH3, and AgM2 designed on the basis of conservative hemagglutinin stem fragments of two influenza A virus subtypes, H1N1 and H3N2, and conservative M2 protein, and evaluate their immunogenicity and protective efficacy.
Article Synopsis
- Researchers are exploring new strategies to create effective vaccines against the Ebola virus due to the current lack of options.
- The study developed two artificial T-cell immunogens that were designed to trigger immune responses and were tested in a mouse model.
- The results indicated that the recombinant plasmids created can produce the necessary mRNAs and proteins, and successfully stimulate T-cell responses, making them promising candidates for future Ebola vaccine development.
This study is focusing on elucidation of the capacity of attenuated Salmonella enteritidis E23 (cya, crp) to serve as a vehicle for the rectal delivery of the DNA vaccine. Earlier for creation HIV-1 candidate DNA vaccine we have designed the polyepitope protein TCI (T-cell immunogen), which comprises over 80 CTL epitopes from subtype A, B and C HIV-1 proteins. The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI.
View Article and Find Full Text PDFGlobal AIDS epidemics caused by human immunodeficiency virus type 1 (HIV-1) has existed for more than 25 years and involved more than 2 million newly infected people annually. The obstacle in combating the global epidemics is a rapid evolution of the virus by the selection of drug resistance mutations. In this review, we have summarized scientific achievements in the field of reverse transcriptase drug resistance to licensed antiviral drugs--nucleoside (NRTI) and non-nucleoside (NNRTI) inhibitors.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates somatic mutations of the p53 gene in stomach cancer patients, utilizing sequential and molecular cloning methods.
- It identified a total of 8 mutations across different tumor types, including a notable single-nucleotide deletion that led to a shifted reading frame and several missense mutations resulting in amino acid changes.
- The research highlights that all mutations appeared in crucial codons, supporting previous findings that tumors display diverse p53 genetic alterations.