[Molecular-genetic polymorphism of barley (Hordeum vulgare L.) cultivars detected with AFLP method].

Analysis of molecular-genetic polymorphism of barley varieties was performed using AFLP-method. A system for identification and differentiation of barley varieties based on AFLP-markers was developed. Results of testing for 19 varieties indicate a high differential ability of the developed system.

[Activity of NAD.H-generating enzymes and cytochrome content in mitochondria from rat liver and myocardium under artificial hypobiosis].

The modification particularities of the structural and functional state of the inner mitochondrial membrane of the rat liver and myocardium were observed in conditions of artificial hypobiosis, which was created using hypoxic and hypercapnic gas medium with a body temperature reduction. Under the artificial hypobiosis the activity of NAD.H-generating enzymes of the Krebs cycle of the liver mitochondria decreases.

[Identification of a pandemic influenza virus A/H1N1 by real-time PCR].

The WHO approved protocol of pandemic influenza virus A/H1N1 identification by the method of one-step RT-PCR was adopted. The cost of research was decreased due to adoption of RNA purification method and utilizing of the common enzyme systems for RT-PCR.

[Bacterial synthesis of the protein G IgG-binding domain of Streptococcus sp. and prospects of its utilization in immunological practice].

The chemical synthesis of a modified gene fragment that codes IgG-binding domain of protein G of Streptococcus sp. (SPG) was done. Two copies of gene fragmentes SPG were cloned into plasmid vector pET-24(a), for expression of recombinant truncated protein G (rSPG) in the culture of Escherichia coli.

[Influence of conditions of artificial hibernation on energy metabolism indices in rats].

Influence of the conditions of artificial hibernation on the content of ATP, ADP, AMP, cAMF in the blood and liver of rats is studied. It is established, that the entering and stay of animals in the conditions of artificial hibernation is accompanied by a decrease of the content of ADP and P(l). The animal coming out of the condition of artificial hibernation is accompanied by an increase of the content of ATP and cAMP and achievement in twenty-four hours of the level of monitoring values.