Adipocyte heterogeneity and tumor infiltration of adipose tissue in patients with metastatic breast cancer.

Background: The adipose tissue may serve as a source of energy supporting cancer growth and metastasis. Our understanding of the adipocytes which compose the adipose tissue in different anatomical locations of the body as well as potential microscopic tumor infiltration in patients with metastatic breast cancer remains limited. This study therefore investigates regional variations in adipocyte size and adipose tissue tumor infiltration in patients with metastatic breast cancer.

Stromal Tumor-Infiltrating Lymphocytes in Hormone Receptor-Positive/HER2 Negative Metastatic Breast Cancer.

The immune landscape of hormone receptor-positive, human epidermal growth factor receptor 2-negative metastatic breast cancer (HR+/HER2- mBC), the most common subtype of BC, remains understudied. This is mainly because of reduced sample acquisition opportunities from metastases as compared with primary tumors. In this study, we explored stromal tumor-infiltrating lymphocytes (sTIL) in metastatic samples collected through our post-mortem tissue donation program UZ/KU Leuven Post-mortem Tissue Donation program to Enhance Research (NCT04531696).

Model linkage to assess forest disturbance impacts on water quality: A wildfire case study using LANDIS(II)-VELMA.

Wildfires in western US forests increased over the last two decades, resulting in elevated solid and nutrient loadings to streams, and occasionally threatening drinking water supplies. We demonstrated that a linked LANDIS (LANDscape DIsturbance and Succession)-VELMA (Visualizing Ecosystem Land Management Assessments) modeling approach can simulate wildland fire effects on water quality using the 2002 Colorado Hayman Fire. Utilizing LANDIS-II's forest landscape model to simulate forest composition and VELMA's eco-hydrologic model to simulate pre- and post-fire water quantity and quality, the best calibration performance yielded a Nash-Sutcliffe Efficiency (NSE) of 0.

Non-viral DNA delivery and TALEN editing correct the sickle cell mutation in hematopoietic stem cells.

Sickle cell disease is a devastating blood disorder that originates from a single point mutation in the HBB gene coding for hemoglobin. Here, we develop a GMP-compatible TALEN-mediated gene editing process enabling efficient HBB correction via a DNA repair template while minimizing risks associated with HBB inactivation. Comparing viral versus non-viral DNA repair template delivery in hematopoietic stem and progenitor cells in vitro, both strategies achieve comparable HBB correction and result in over 50% expression of normal adult hemoglobin in red blood cells without inducing β-thalassemic phenotype.

A systematic scoping review of the latest data on orthobiologics in the surgical treatment of non-union.

Introduction: Recent studies have shown a growing concern regarding the cost-effectiveness and the lack of supporting data for the biologic agents that are being increasingly used in the orthopedic field. Our aim was to conduct a systematic scoping review of recent publications (last five years) on the use of orthobiologics to treat fracture non-union and summarize the latest available data.

Patients And Methods: The inclusion criteria for this review were articles published in English, from 2016 to 2022, and focusing on the use of orthobiologics for the surgical treatment of non-union.