Publications by authors named "S D Hartson"
Dysregulated muscle glycogen metabolism preslaughter contributes to aberrant postmortem muscle pH (>5.8) in dark-cutting beef phenotypes. However, the underlying mechanisms have remained elusive.
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- Extracellular vesicles (EVs), like exosomes, are important for how cells communicate and control processes in other cells.
- The study focused on Mycobacterium abscessus, a non-tuberculous bacterium that can lead to serious lung infections, particularly in individuals with pre-existing lung issues.
- Researchers found that infection with M. abscessus increased the release of specific exosomes from mouse macrophages, which affected protein content and promoted bacterial growth while lowering glutamine levels, suggesting that these exosomes hinder macrophage ability to kill the bacteria by eliminating glutamine.
Many techniques exist for the identification of protein interaction networks. We present a protocol that relies on an affinity purification-mass spectrometry (AP-MS) approach to detect proteins that co-purify with a tagged bait of interest from Drosophila melanogaster larval muscles using the GAL4/upstream activating sequence (UAS) expression system. We also describe steps for the isolation and identification of protein complexes, followed by streamlined bioinformatics analysis for rapid and reproducible results.
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- Anaerobic gut fungi (AGF) are mostly found in the gastrointestinal tracts of herbivores, and while their role in mammals is well-known, their presence in non-mammals like tortoises is less documented.
- Research indicates that tortoises host a unique AGF community with three new genera, estimated to have diverged from their ancestors over 104-112 million years ago, suggesting an early evolution of symbiosis between animals and AGF.
- Tortoise-associated AGF show limited ability to metabolize plant materials due to fewer carbohydrate-active enzymes and reduced gene exchange compared to AGF from mammals, leading to a diminished capacity for breaking down cellulose.
The analysis of the secretome allows us to identify the proteins, especially carbohydrate-active enzymes (CAZymes), secreted by different microorganisms cultivated under different conditions. The CAZymes are divided into five classes containing different protein families. is a thermophilic ascomycete, a source of many glycoside hydrolases and oxidative enzymes that aid in the breakdown of lignocellulosic materials.
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