Publications by authors named "S D Harbaugh"
Article Synopsis
- * The study introduces Sensor-seq, a high-throughput platform that allows for the design and identification of aTF biosensors that can bind to a variety of non-native ligands, testing 17,737 variants of aTF TtgR against multiple compounds.
- * Sensor-seq successfully finds biosensors with high performance for various ligands and showcases their practical use through cell-free detection systems for specific drugs, enhancing the ability to create new biosensors beyond natural limitations.
Implantable sensors that can monitor analytes related to cognitive and physiological status have gained significant focus in recent years. We have developed an implantable biosensor to detect dehydroepiandrosterone sulfate (DHEA-S), a biomarker related to stress. The biosensor strategy was based on the principle of forced intercalation (FIT) aptamers designed to detect subtle intramolecular changes during aptamer-target binding events.
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- Allosteric transcription factors (aTFs) are important tools for detecting various molecules but designing them to work with new compounds is difficult due to potential disruptions in their allosteric properties.
- The researchers developed a high-throughput platform called Sensor-seq that screened over 17,000 variants of the aTF TtgR to find biosensors that can bind to a range of non-native and native ligands.
- The study resulted in the identification of new biosensors with excellent specificity and effectiveness, and practical applications were demonstrated through cell-free detection systems for naltrexone and quinine.
A ratiometric response gives an output that is proportional to the ratio between the magnitudes of two inputs. Ratio computation has been observed in nature and is also needed in the development of smart probiotics and organoids. Here, we achieve ratiometric gene expression response in bacteria Escherichia coli with the incoherent merger network.
View Article and Find Full Text PDFThe detection of chemicals using natural allosteric transcription factors is a powerful strategy for point-of-use molecular sensing, particularly using fieldable cell-free gene expression (CFE) systems. However, the reliance of detection schemes on characterized protein-based sensors limits the number of measurable analytes. One alternative solution to this issue is to develop new sensors by generating RNA aptamers against the target analyte and then incorporating them directly into a riboswitch scaffold for ligand-inducible genetic control of a reporter protein.
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